Journal: Emerging Infectious Diseases

Article Title: Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus

doi: 10.3201/eid2302.161239

Figure Lengend Snippet: Presence of MERS-CoV receptor DPP4 (IHC) and of mucosubstances (PAS) in upper and lower respiratory tract tissues from sheep, pigs, llamas, and horses. A) In the nose, DPP4 (red cytoplasmic or membrane staining) was present on the lining epithelium of pigs, llamas, and horses but not sheep. PAS staining (magenta) demonstrated more mucous cells in the lining epithelium of sheep and horses and a layer of mucus on the lining epithelium of the horses. B) DPP4 (red cytoplasmic or membrane staining) was present on the lining epithelium of the trachea, bronchus/bronchioles, and alveoli in the pigs, llamas and horses but not in the sheep. Original magnification ×400 for all images. DPP4, dipeptidyl peptidase-4; IHC, immunohistochemistry; MERS-CoV, Middle East respiratory syndrome coronavirus; PAS, periodic acid–Schiff; term., terminal.

Article Snippet: In brief, we performed DPP4 IHC staining by using 5 μg/mL of polyclonal goat IgG anti-human DPP4 antibody (R&D Systems, Abingdon, UK) and peroxidase-labeled rabbit anti-goat IgG (1:200; DAKO; Agilent Technologies Company, Santa Clara, CA, USA) as a secondary antibody.

Techniques: Membrane, Staining, Immunohistochemistry

Journal: Journal of Pediatric Hematology/Oncology

Article Title: Chemotherapy-induced Alteration of SDF-1/CXCR4 Expression in Bone Marrow–derived Mesenchymal Stem Cells From Adolescents and Young Adults With Acute Lymphoblastic Leukemia

doi: 10.1097/mph.0000000000000220

Figure Lengend Snippet: FIGURE 3. Secretion and expression of SDF-1 in BM-MSCs. A, SDF-1 level was measured by enzyme-linked immunosorbent assay. (a) The bone marrow plasma level of SDF-1 in patients with ALL was significantly increased compared with that in healthy donors and ALL-c (*P < 0.05; n = 10). (b), The same difference was found when SDF-1 levels were measured in the supernatants of MSC cultures after incubation for 72 hours. B, Western blotting was performed to quantify total SDF-1 in the lysates of MSCs. SDF-1 expression in the MSCs of ALL was significantly higher than that in the MSCs of healthy donors and ALL-c. C, The difference was also found at the transcriptional level by real-time PCR (*P < 0.05, n = 10). D, Surface-CD26 expression on the MSCs from ALL patients measured by flow cytometry was significantly lower than that in healthy donors and ALL-c (*P < 0.05; n = 10). E, Total CD26 was measured by Western blot in the MSCs. The fold difference was shown among the 3 types of MSCs. F, The same difference was also found at the transcriptional level (*P < 0.05; n = 10). ALL indicates acute lymphoblastic leukemia; BM-MSC, bone marrow–derived mesenchymal stem cell; GADPH, glyceraldehyde- 3-phosphate dehydrogenase; PCR, polymerase chain reaction.

Article Snippet: Membranes were hybridized with the following primary antibodies: rabbit anti-human CXCR4 (1:1000; Abcam), goat antihuman SDF-1(1:500; R&D Systems), goat anti-human CD26 (1:1000; R&D Systems), and mouse antibody to glyceraldehyde-3-phosphate dehydrogenase (1:4000; Santa Cruz).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Incubation, Western Blot, Real-time Polymerase Chain Reaction, Flow Cytometry, Derivative Assay, Polymerase Chain Reaction

Journal: International Journal of Cardiology

Article Title: Cardiac inflammation and microvascular procoagulant changes are decreased in second wave compared to first wave deceased COVID-19 patients

doi: 10.1016/j.ijcard.2021.11.079

Figure Lengend Snippet: Comparison of coagulation regulating factors and CML in the cardiac microvasculature between first and second wave COVID-19 and control patients. The number of blood vessels positive for the procoagulant factors Tissue Factor (A; TF), Factor VII (B; FVII), Factor XII (C; FXII) and anticoagulant dipeptidyl peptidase 4 (D; DPP4) are shown per cm 2 of left ventricular heart tissue in control patients (Con; n = 18) and first wave (Wave 1; n = 15) and second wave (Wave 2; n = 10) COVID-19 patients. (E): An immunohistochemical example of the presence N(ε)-Carboxymethyllysine (CML) in the endothelium of intramyocardial blood vessels of a COVID-19 patient (arrow) and the immunohistochemical (IH) score for CML per cm 2 in control patients (Con) and first- and second wave COVID-19 patients. (F): The number of blood vessels with weak, moderate and strong CML staining (staining intensities 1, 2, 3 respectively) in control (Con) and first- and second wave COVID-19 patients. Each point in the graphs represents the value of one individual patient, the bars represent mean ± SD. The bars represent mean ± SD. For comparisons Kruskal-Wallis tests with Dunn's multiple comparison tests were used. *p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001 (exact p-values are given in the text).

Article Snippet: Primary antibodies were added for 1 h at room temperature (RT): mouse-anti-human CD45 (1:100, Dako Santa Clara, USA; M0701), rabbit-anti-human CD68 (1:400, Dako; M0814), rabbit-anti-human CD3 (1:100, Dako; A0452), rabbit-anti-human C3d (1:1000, Dako; A0063), mouse-anti-human CD31 (1:50, Dako; M0823), mouse-anti-human TF (1:250, Biorbyt Cambridge, UK; ORB100189), mouse-anti-human FVII (1:100, Sanquin Research, Amsterdam, The Netherlands), mouse-anti-human FXII (1:25, Sanquin), mouse-anti-human CML (1:500 [ ]) or mouse-anti-human CD26 (1:100, Bio-Rad, Lunteren, The Netherlands, MCA1317T).

Techniques: Comparison, Coagulation, Control, Immunohistochemical staining, Staining

Journal: bioRxiv

Article Title: Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes

doi: 10.1101/2021.01.17.427024

Figure Lengend Snippet: A) ACE2 expressing cells are readily infected by SARS-CoV-2, where they have abundant ACE2 and dsRNA presence. Primary cortical tissue and cortical organoids do not have observable ACE2 protein in cortical tissue or infected cells. NRP1 is present in cortical neurons, but not in infected cells. B) Infected cortical astrocytes in the SVZ of primary cortex abundantly express coronavirus receptors DPP4 and CD147, where 100% of cells assayed have DPP4 and 73% have CD147. In cortical organoids, 60% are DPP4+ and 28% are CD147+ (Primary: DPP4 n=61cells, CD147 n=83 cells; Organoid: DPP4 n=296 cells, CD147 n=239 cells) C) Inhibition of DPP4 by Vildagliptin results in a 30% decrease in the number of SARS-CoV-2 N+ cells and a 70% reduction in dsRNA+ cells. SARS-CoV-2 N+ dsRNA+ cells are indicated by white arrowheads (SARS-CoV-2 MOI 0.5 n=1273 cells, MOI 0.5+Vildagliptin n=879 cells, dsRNA MOI 0.5 n=571 cells, MOI 0.5+Vildagliptin n=227 cells across two biological samples and three technical replicates). D) ARCN1 is broadly expressed in SARS-CoV-2 infected samples, which is reduced by 70% after DPP4 inhibition by Vildagliptin. ARCN1+ dsRNA+ cells indicated by white arrowheads (MOI 0.5 n=1224 cells, MOI 0.5+ Vildagliptin n=331 cells across two biological samples and three technical replicates).

Article Snippet: Primary Antibodies include: Mouse: dsRNA, clone rJ2 (Millipore, MABE1134, 1:100), Sox2 (Santa Cruz, sc-365823, 1:500), S100B (Sigma, S2532, 1:200), Ki67 (Abcam, ab156956, 1:500), CD31 (Agilent, GA61061–2, 1:100), Olig2 (Millipore, MABN50, 1:100), Rabbit: SARS-CoV-2 (Sino Biological, 40143-R001, 1:200), Pax6 (Biolegend, 901301, 1:500), Hopx (Proteintech, 11419–1-AP, 1:500), Cleaved Caspase-3 (Cell Signaling, 9661S, 1:250), Synm (Proteintech, 20735–1-AP, 1:100), Aqp4 (Proteintech, 16473–1-AP, 1:600), Egfr (Abcam, ab32077, 1:100), Dpp4 (Proteintech, 10940–1-AP, 1:50), CD147 (Invitrogen, 34–5600, 1:500), Arcn1 (Proteintech, 23843–1-AP, 1:50), Rat: Gfap (Thermofisher, 13–0300, 1:200), Laminin (Abcam, ab80580, 1:500), Nrp1 (Abcam, ab81321, 1:50), Chicken: Gfap (Abcam, ab4674, 1:500), Map2 (Abcam, ab5392, 1:200), Goat: Ace2 (R&D, AF933, 1:200), Ace2 (Thermofisher, MA5–32307, 1:200), Iba1 (Abcam, ab48004, 1:500), Pdgfrb (R&D, AF385, 1:100), Sheep: Eomes (R&D, AF6166, 1:200), Guinea pig: NeuN (Millipore, ABN90, 1:500), Sheep: CD34 (R&D, AF7227, 1:200).

Techniques: Expressing, Infection, Inhibition

Journal: bioRxiv

Article Title: Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes

doi: 10.1101/2021.01.17.427024

Figure Lengend Snippet:

Article Snippet: Primary Antibodies include: Mouse: dsRNA, clone rJ2 (Millipore, MABE1134, 1:100), Sox2 (Santa Cruz, sc-365823, 1:500), S100B (Sigma, S2532, 1:200), Ki67 (Abcam, ab156956, 1:500), CD31 (Agilent, GA61061–2, 1:100), Olig2 (Millipore, MABN50, 1:100), Rabbit: SARS-CoV-2 (Sino Biological, 40143-R001, 1:200), Pax6 (Biolegend, 901301, 1:500), Hopx (Proteintech, 11419–1-AP, 1:500), Cleaved Caspase-3 (Cell Signaling, 9661S, 1:250), Synm (Proteintech, 20735–1-AP, 1:100), Aqp4 (Proteintech, 16473–1-AP, 1:600), Egfr (Abcam, ab32077, 1:100), Dpp4 (Proteintech, 10940–1-AP, 1:50), CD147 (Invitrogen, 34–5600, 1:500), Arcn1 (Proteintech, 23843–1-AP, 1:50), Rat: Gfap (Thermofisher, 13–0300, 1:200), Laminin (Abcam, ab80580, 1:500), Nrp1 (Abcam, ab81321, 1:50), Chicken: Gfap (Abcam, ab4674, 1:500), Map2 (Abcam, ab5392, 1:200), Goat: Ace2 (R&D, AF933, 1:200), Ace2 (Thermofisher, MA5–32307, 1:200), Iba1 (Abcam, ab48004, 1:500), Pdgfrb (R&D, AF385, 1:100), Sheep: Eomes (R&D, AF6166, 1:200), Guinea pig: NeuN (Millipore, ABN90, 1:500), Sheep: CD34 (R&D, AF7227, 1:200).

Techniques: Sequencing

Journal: Oncotarget

Article Title: The associations of genetic polymorphisms in CYP1A2 and CYP3A4 with clinical outcomes of breast cancer patients in northern China

doi: 10.18632/oncotarget.16359

Figure Lengend Snippet: Staining for each specimen is shown at two magnification: top, 100×; bottom, 400×. CYP1A2 protein low expression specimens ( A , B ); CYP1A2 protein high expression specimens ( C , D ).

Article Snippet: The sections were incubated overnight at 4°C with primary antibody CYP1A2 (1:100 dilution, a recombinant rabbit monoclonal antibody, BOSTER: PB0574) and CYP3A4 (1:50 dilution, a recombinant rabbit monoclonal antibody, BOSTER: PB1111).

Techniques: Staining, Expressing

Journal: Oncotarget

Article Title: The associations of genetic polymorphisms in CYP1A2 and CYP3A4 with clinical outcomes of breast cancer patients in northern China

doi: 10.18632/oncotarget.16359

Figure Lengend Snippet: Association of CYP1A2 and CYP3A4 genetic polymorphisms with protein expression

Article Snippet: The sections were incubated overnight at 4°C with primary antibody CYP1A2 (1:100 dilution, a recombinant rabbit monoclonal antibody, BOSTER: PB0574) and CYP3A4 (1:50 dilution, a recombinant rabbit monoclonal antibody, BOSTER: PB1111).

Techniques: Expressing

Journal: Oncotarget

Article Title: The associations of genetic polymorphisms in CYP1A2 and CYP3A4 with clinical outcomes of breast cancer patients in northern China

doi: 10.18632/oncotarget.16359

Figure Lengend Snippet: Prognostic factors in the cox proportional hazards model

Article Snippet: The sections were incubated overnight at 4°C with primary antibody CYP1A2 (1:100 dilution, a recombinant rabbit monoclonal antibody, BOSTER: PB0574) and CYP3A4 (1:50 dilution, a recombinant rabbit monoclonal antibody, BOSTER: PB1111).

Techniques: